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Frequently Asked Questions

How long does it take an autoclave to sterilize goods?

How long it takes to sterilize a load depends entirely on the content of the load, the set temperature of the autoclave, and the Sterility Assurance Level desired. Generally speaking, the higher the temperature, the faster a load will achieve sterility.

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How does an autoclave sterilize medical devices?

Autoclaves use extreme heat in the form of pressurized steam to sterilize goods. Similar to a pressure cooker, an autoclave uses a locked door to create a sealed chamber. The air within that chamber is then replaced by steam, which is pressurized until the goods within the chamber have been sufficiently sterilized.

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What temperature(s) can an autoclave reach?

Autoclaves are typically designed to reach temperatures between 250°F and 275°F (121°C and 135°C).

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How do I ensure proper steam penetration and drying for bulky loads?

Proper load arrangement and cycle selection are key. Autoclaves with vacuum cycles remove air pockets, ensuring steam reaches all surfaces. A built-in drying phase prevents moisture buildup and bacterial growth.

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What are the phases of sterilization?

The general process for using an autoclave to sterilize goods breaks down into three basic phases: The purge phase, during which steam displaces air within the autoclave chamber and temperature and pressure steadily increases The exposure (sterilization) phase, during which the autoclave’s control program closes the exhaust valve, causing the autoclave’s interior temperature and pressure […]

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How frequently should medical devices be autoclaved?

Medical devices should always be sterilized after each use. Items packaged in dry double-wrap linen packs or an inner layer of paper and an outer layer of plastic are proven to remain sterile for up to 96 weeks.

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How long do items stay sterile after autoclaving?

It depends entirely on how items are packaged after sterilization. Generally speaking, items should be re-sterilized after each use, but items packaged in double-wrap linen packs or an inner layer of paper and outer layer of plastic have been proven to remain sterile for up to 96 weeks.

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What are the different types of autoclaves?

The two most common types of steam sterilizers are gravity displacement autoclaves and high-speed prevacuum autoclaves. Both types of autoclave come in a variety of shapes and sizes, ranging from tabletop units to room-sized bulk units, with a vast array of customization options, such as vertical sliding doors, double-door pass-thru chambers, and stackable dual chambers.

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How do you sterilize membrane filters?

Membrane filters are typically sterilized by autoclaving at temperatures between 121–134°C. To prevent damage, place filters in protective casing and position them within the autoclave chamber to allow for full steam penetration while avoiding mechanical stress. Custom cycle settings, such as reduced pressure ramp rates and controlled drying, can preserve membrane integrity.

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What is the process of sterilization in bioprocess?

Biopharmaceutical facilities can use steam sterilization to eliminate all viable microorganisms from components that come into contact with process fluids, such as bioprocessing filters, tubing, and vessels. Steam sterilization, also known as autoclaving, applies time, temperature, and saturated pressure to ensure microbial inactivation in accordance with the sterility assurance levels required by GMP/cGMP guidelines.

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12.10.25

AAMI ST108 Compliance

Introducing SterilSteam Sampler, The Steam Condensate Sampler for AAMI ST108 Compliance →

Released in 2023, AAMI/ANSI ST108 establishes water quality requirements for water used in the cleaning, rinsing, disinfection, and sterilization of reusable medical devices. In addition to these requirements, ST108 mandates quarterly testing of steam condensate quality to ensure incoming steam does not stain instruments or pose a risk to patient safety. To help healthcare facilities […]

11.6.25

Critical Water for Autoclaves

AAMI ST108 & Critical Water for Autoclaves: What ASCs Need to Know →

A major step forward in patient safety, ANSI/AAMI ST108 establishes new minimum water quality requirements for water used in the cleaning, rinsing, disinfection, and sterilization of reusable medical devices. Despite providing guidance on water quality management for sterile processing, ST108 has also created confusion, especially regarding test protocols, water treatment, and critical water requirements. In […]

10.20.25

woman with long dark hair wearing a white lab coat and blue rubber gloves removes trays from an autoclave

What Is an Autoclave Machine Really Used for? [Applications, Costs & More] →

Autoclave machines are mission-critical equipment in a variety of industries, from healthcare to biotech, ensuring equipment and materials are sterilized and safe for use. Whether you’re considering purchasing an autoclave for a new facility, replacing a broken one, or expanding your current sterilization capacity, understanding the ins and outs of autoclave technology is key to […]

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